Difference between revisions of "Molecular pathology"
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==Molecular== | ==Molecular== | ||
General: | General: | ||
*Very small changes. | *Very small changes - submicroscopic. | ||
*Sequence data. | |||
Techniques: | Techniques: | ||
*DNA sequencing - | *DNA sequencing. | ||
**Real time-PCR, [[AKA]] real time-quantitative PCR (RQ-PCR). | |||
*RNA sequencing. | *RNA sequencing. | ||
**May be examined after reverse transcription (RNA -> DNA), i.e. RT-PCR. | |||
*Southern blot. | *Southern blot. | ||
**Analysis of proteins. | |||
===Tests=== | ===Tests=== | ||
| Line 27: | Line 31: | ||
*Large changes (chromosomal). | *Large changes (chromosomal). | ||
**Maximum resolution 3-4 megabase pairs (3-4 million base pairs); may be less - dependent on band density.<ref>{{Ref WMSP|695}}</ref> | **Maximum resolution 3-4 megabase pairs (3-4 million base pairs); may be less - dependent on band density.<ref>{{Ref WMSP|695}}</ref> | ||
*Morphologic data. | |||
Techniques: | Techniques: | ||
Revision as of 18:59, 3 May 2011
Molecular pathology is the future of pathology.
Overview
Molecular pathology can be divided as follows:
| Molecular pathology | |||||||||||||||||||
| Molecular techniques | Cytogenetics | ||||||||||||||||||
Molecular
General:
- Very small changes - submicroscopic.
- Sequence data.
Techniques:
- DNA sequencing.
- Real time-PCR, AKA real time-quantitative PCR (RQ-PCR).
- RNA sequencing.
- May be examined after reverse transcription (RNA -> DNA), i.e. RT-PCR.
- Southern blot.
- Analysis of proteins.
Tests
A list of tests are found in the Molecular pathology tests article.
Cytogenetics
General:
- Large changes (chromosomal).
- Maximum resolution 3-4 megabase pairs (3-4 million base pairs); may be less - dependent on band density.[1]
- Morphologic data.
Techniques:
- ISH = in situ hybridization.
- FISH = fluorescent in situ hybridization.
- SISH = silver in situ hybridization.[2]
Image:
World protein databank
I can't help think it is ironic that the protein databank goal is to maintain a free and publicly available archive,[3] yet the announcement is in pay-for-access journal (Nature Structual Biology).[4]
Wnt/beta-catenin pathway
Important in hepatoblastomas.[5]
See also
References
- ↑ Humphrey, Peter A; Dehner, Louis P; Pfeifer, John D (2008). The Washington Manual of Surgical Pathology (1st ed.). Lippincott Williams & Wilkins. pp. 695. ISBN 978-0781765275.
- ↑ URL: http://www.immunoportal.com/modules.php?name=News&file=article&sid=186. Accessed on: 2 May 2011.
- ↑ Worldwide Protein Data Bank. URL: http://www.wwpdb.org/faq.html Accessed on: April 22, 2009.
- ↑ Berman H, Henrick K, Nakamura H (December 2003). "Announcing the worldwide Protein Data Bank". Nat. Struct. Biol. 10 (12): 980. doi:10.1038/nsb1203-980. PMID 14634627.
- ↑ Cotran, Ramzi S.; Kumar, Vinay; Fausto, Nelson; Nelso Fausto; Robbins, Stanley L.; Abbas, Abul K. (2005). Robbins and Cotran pathologic basis of disease (7th ed.). St. Louis, Mo: Elsevier Saunders. pp. 923. ISBN 0-7216-0187-1.