Difference between revisions of "HPFitis"
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'''HPFitis''' is use of ''HPF'' (high-power field) without proper qualification, i.e. without giving the field area. | '''HPFitis''' is use of ''HPF'' (high-power field) without proper qualification, i.e. without giving the field area. | ||
''HPF'' redirects here. | |||
===Etiology=== | |||
Its etiology is thought to be either: | Its etiology is thought to be either: | ||
#Ignorance of statistics and sampling theory. | #Ignorance of statistics and sampling theory. | ||
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It appears to have a higher incidence in math phobic individuals. | It appears to have a higher incidence in math phobic individuals. | ||
===Consequences of HPFitis=== | |||
*Papers on [[eosinophilic esophagitis]] are ''not'' comparable.<ref name=pmid17617209>{{cite journal |author=Dellon ES, Aderoju A, Woosley JT, Sandler RS, Shaheen NJ |title=Variability in diagnostic criteria for eosinophilic esophagitis: a systematic review |journal=Am. J. Gastroenterol. |volume=102 |issue=10 |pages=2300–13 |year=2007 |month=October |pmid=17617209 |doi=10.1111/j.1572-0241.2007.01396.x |url=}}</ref> | |||
*[[GIST]]s were improperly [[cancer staging|staged]] - until they standardized to 5 mm<sup>2</sup>. | |||
*Mitotic score in the Nottingham score for [[invasive breast cancer]] is based on the pseudo-standard area of "10 HPFs"; this results in significant variance due to the field area of the microscope in large cohorts.<ref name=pmid28202066>{{Cite journal | last1 = Bonert | first1 = M. | last2 = Tate | first2 = AJ. | title = Mitotic counts in breast cancer should be standardized with a uniform sample area. | journal = Biomed Eng Online | volume = 16 | issue = 1 | pages = 28 | month = Feb | year = 2017 | doi = 10.1186/s12938-016-0301-z | pmc = 5312435 | PMID = 28202066 }}</ref> | |||
===Field area=== | |||
Most modern [[microscope]]s, have an eye piece diameter of 22 mm. Therefore, the field diameter at 40 X is approximately 22 mm / 40 X ~= 0.55 mm and the field of view is pi/4*(0.55 mm)<sup>2</sup> = 0.2376 mm<sup>2</sup>. | |||
==See also== | ==See also== | ||
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*[[IPFitis]] - a related uncommon disease. | *[[IPFitis]] - a related uncommon disease. | ||
*[[Basics]]. | *[[Basics]]. | ||
==References== | |||
{{Reflist|1}} | |||
[[Category:Rants]] | [[Category:Rants]] | ||
Latest revision as of 14:52, 26 November 2021
HPFitis is use of HPF (high-power field) without proper qualification, i.e. without giving the field area.
HPF redirects here.
Etiology
Its etiology is thought to be either:
- Ignorance of statistics and sampling theory.
- Laziness.
- A combination of the above two.
It appears to have a higher incidence in math phobic individuals.
Consequences of HPFitis
- Papers on eosinophilic esophagitis are not comparable.[1]
- GISTs were improperly staged - until they standardized to 5 mm2.
- Mitotic score in the Nottingham score for invasive breast cancer is based on the pseudo-standard area of "10 HPFs"; this results in significant variance due to the field area of the microscope in large cohorts.[2]
Field area
Most modern microscopes, have an eye piece diameter of 22 mm. Therefore, the field diameter at 40 X is approximately 22 mm / 40 X ~= 0.55 mm and the field of view is pi/4*(0.55 mm)2 = 0.2376 mm2.
See also
References
- ↑ Dellon ES, Aderoju A, Woosley JT, Sandler RS, Shaheen NJ (October 2007). "Variability in diagnostic criteria for eosinophilic esophagitis: a systematic review". Am. J. Gastroenterol. 102 (10): 2300–13. doi:10.1111/j.1572-0241.2007.01396.x. PMID 17617209.
- ↑ Bonert, M.; Tate, AJ. (Feb 2017). "Mitotic counts in breast cancer should be standardized with a uniform sample area.". Biomed Eng Online 16 (1): 28. doi:10.1186/s12938-016-0301-z. PMC 5312435. PMID 28202066. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312435/.