Difference between revisions of "Molecular pathology"
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*Southern blot. | *Southern blot. | ||
**Analysis of proteins. | **Analysis of proteins. | ||
*Amplification-refractory mutation system (ARMS):<ref name=pmid18428319>{{cite journal |author=Little S |title=Amplification-refractory mutation system (ARMS) analysis of point mutations |journal=Curr Protoc Hum Genet |volume=Chapter 9 |issue= |pages=Unit 9.8 |year=2001 |month=May |pmid=18428319 |doi=10.1002/0471142905.hg0908s07 |url=}}</ref> | |||
**Technique for finding a (specific) single base change. | |||
***The (PCR) primers are designed bind to mutated sequence. | |||
****If the mutation is present a PCR product is seen. | |||
****If the mutation is absent no PCR product is seen. | |||
===Tests=== | ===Tests=== | ||
Revision as of 14:19, 4 May 2011
Molecular pathology is the future of pathology.
Overview
Molecular pathology can be divided as follows:
| Molecular pathology | |||||||||||||||||||
| Molecular techniques | Cytogenetics | ||||||||||||||||||
Molecular
General:
- Very small changes - submicroscopic.
- Sequence data.
Techniques:
- DNA sequencing.
- Real time-PCR, AKA real time-quantitative PCR (RQ-PCR).
- RNA sequencing.
- May be examined after reverse transcription (RNA -> DNA), i.e. RT-PCR.
- Southern blot.
- Analysis of proteins.
- Amplification-refractory mutation system (ARMS):[1]
- Technique for finding a (specific) single base change.
- The (PCR) primers are designed bind to mutated sequence.
- If the mutation is present a PCR product is seen.
- If the mutation is absent no PCR product is seen.
- The (PCR) primers are designed bind to mutated sequence.
- Technique for finding a (specific) single base change.
Tests
A list of tests are found in the Molecular pathology tests article.
DNA & RNA extraction
- Techniques are largely standardized.
- Protocols exist for fresh tissue and formulin fixed parafin imbeded tissue.
Cytogenetics
General:
- Large changes (chromosomal).
- Maximum resolution 3-4 megabase pairs (3-4 million base pairs); may be less - dependent on band density.[4]
- Morphologic data.
Techniques:
- ISH = in situ hybridization.
- FISH = fluorescent in situ hybridization.
- SISH = silver in situ hybridization.[5]
Image:
World protein databank
I can't help think it is ironic that the protein databank goal is to maintain a free and publicly available archive,[6] yet the announcement is in pay-for-access journal (Nature Structual Biology).[7]
Wnt/beta-catenin pathway
Important in hepatoblastomas.[8]
See also
References
- ↑ Little S (May 2001). "Amplification-refractory mutation system (ARMS) analysis of point mutations". Curr Protoc Hum Genet Chapter 9: Unit 9.8. doi:10.1002/0471142905.hg0908s07. PMID 18428319.
- ↑ Chomczynski P, Sacchi N (2006). "The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on". Nat Protoc 1 (2): 581–5. doi:10.1038/nprot.2006.83. PMID 17406285.
- ↑ Pikor LA, Enfield KS, Cameron H, Lam WL (2011). "DNA extraction from paraffin embedded material for genetic and epigenetic analyses". J Vis Exp (49). doi:10.3791/2763. PMID 21490570.
- ↑ Humphrey, Peter A; Dehner, Louis P; Pfeifer, John D (2008). The Washington Manual of Surgical Pathology (1st ed.). Lippincott Williams & Wilkins. pp. 695. ISBN 978-0781765275.
- ↑ URL: http://www.immunoportal.com/modules.php?name=News&file=article&sid=186. Accessed on: 2 May 2011.
- ↑ Worldwide Protein Data Bank. URL: http://www.wwpdb.org/faq.html Accessed on: April 22, 2009.
- ↑ Berman H, Henrick K, Nakamura H (December 2003). "Announcing the worldwide Protein Data Bank". Nat. Struct. Biol. 10 (12): 980. doi:10.1038/nsb1203-980. PMID 14634627.
- ↑ Cotran, Ramzi S.; Kumar, Vinay; Fausto, Nelson; Nelso Fausto; Robbins, Stanley L.; Abbas, Abul K. (2005). Robbins and Cotran pathologic basis of disease (7th ed.). St. Louis, Mo: Elsevier Saunders. pp. 923. ISBN 0-7216-0187-1.