Difference between revisions of "Tissue processing"
Jump to navigation
Jump to search
(→See also: +link to molecular pathol.) |
m (wikify, format) |
||
| Line 3: | Line 3: | ||
==What happens to the tissue== | ==What happens to the tissue== | ||
In an ideal world the pathologist gets the tissue fresh and divides it up in the following way:<ref>PST. 14 February 2011.</ref> | In an ideal world the pathologist gets the tissue fresh and divides it up in the following way:<ref>PST. 14 February 2011.</ref> | ||
# | #[[Formalin]]. | ||
#Electron microscopy. | #[[Electron microscopy]]. | ||
#Molecular genetics. | #Molecular genetics. | ||
#Cytogenetics. | #[[Cytogenetics]]. | ||
#Flow cytometery. | #[[Flow cytometery]]. | ||
#Tissue banks - institutional, regional, national, international. | #Tissue banks - institutional, regional, national, international. | ||
#Tissue culture. | #Tissue culture. | ||
| Line 13: | Line 13: | ||
==Tissue processing== | ==Tissue processing== | ||
Basic process: | Basic process: | ||
#Fixation -- | #Fixation -- formalin. | ||
#Embedding -- replace water (in the tissue) with (paraffin) wax - using a series of alcohols. | #Embedding -- replace water (in the tissue) with (paraffin) wax - using a series of alcohols. | ||
#Sectioning -- cut with microtome ~ 3-10 micrometers thick, routinue is usu. 6-8 micrometers.<ref>URL: [http://library.med.utah.edu/WebPath/HISTHTML/HISTOTCH/HISTOTCH.html http://library.med.utah.edu/WebPath/HISTHTML/HISTOTCH/HISTOTCH.html]. Accessed on: 7 March 2011.</ref> | #Sectioning -- cut with microtome ~ 3-10 micrometers thick, routinue is usu. 6-8 micrometers.<ref>URL: [http://library.med.utah.edu/WebPath/HISTHTML/HISTOTCH/HISTOTCH.html http://library.med.utah.edu/WebPath/HISTHTML/HISTOTCH/HISTOTCH.html]. Accessed on: 7 March 2011.</ref> | ||
| Line 23: | Line 23: | ||
==References== | ==References== | ||
{{Reflist| | {{Reflist|2}} | ||
==External links== | ==External links== | ||
Revision as of 13:42, 24 May 2011
Tissue processing is a key component of the histologic examination. This article covers it superficially. It is something a pathologist should have a rudimentary understanding of.
What happens to the tissue
In an ideal world the pathologist gets the tissue fresh and divides it up in the following way:[1]
- Formalin.
- Electron microscopy.
- Molecular genetics.
- Cytogenetics.
- Flow cytometery.
- Tissue banks - institutional, regional, national, international.
- Tissue culture.
Tissue processing
Basic process:
- Fixation -- formalin.
- Embedding -- replace water (in the tissue) with (paraffin) wax - using a series of alcohols.
- Sectioning -- cut with microtome ~ 3-10 micrometers thick, routinue is usu. 6-8 micrometers.[2]
- Staining.
See also
References
- ↑ PST. 14 February 2011.
- ↑ URL: http://library.med.utah.edu/WebPath/HISTHTML/HISTOTCH/HISTOTCH.html. Accessed on: 7 March 2011.