Difference between revisions of "Celiac sprue"

From Libre Pathology
Jump to navigation Jump to search
(→‎TTG result not available: tweak a bit + add obesity)
 
(72 intermediate revisions by 2 users not shown)
Line 1: Line 1:
'''Celiac sprue''' is a common pathology that affects the [[duodenum]].  
{{ Infobox diagnosis
| Name      = {{PAGENAME}}
| Image      = Celiac disease - high mag.jpg
| Width      =
| Caption    = Celiac disease. [[H&E stain]].
| Synonyms  = celiac disease
| Micro      = Intraepithelial lymphocytes +/- villous blunting
| Subtypes  =
| LMDDx      = [[Giardiasis]], [[Enteropathy-associated T-cell lymphoma]] ([[EATL]]), [[inflammatory bowel disease]], [[MALT lymphoma]], others
| Stains    =
| IHC        =
| EM        =
| Molecular  =
| IF        =
| Gross      =
| Grossing  =
| Site      = [[Duodenum]]
| Assdx      = [[dermatitis herpetiformis]], IgA deficiency, [[EATL]], [[duodenal adenocarcinoma]]
| Syndromes  =
| Clinicalhx = improves with gluten free diet
| Signs      = diarrhea
| Symptoms  =
| Prevalence = uncommon
| Bloodwork  = TTG elevated (>10 U/mL)
| Rads      =
| Endoscopy  =
| Prognosis  =
| Other      =
| ClinDDx    = [[normal duodenum]]
| Tx        = gluten free diet
}}
'''Celiac sprue''', also '''celiac disease''' (abbreviated '''CD'''), is a common pathology that affects the [[duodenum]].
 
It should not be confused with ''tropical sprue''.


An introduction to gastrointestinal pathology is in the ''[[gastrointestinal pathology]]'' article.  It covers basic gastrointestinal histology.
An introduction to gastrointestinal pathology is in the ''[[gastrointestinal pathology]]'' article.  It covers basic gastrointestinal histology.


==Etiology==
==General==
*Autoimmune.
*Considered an autoimmune disease.
*The typical individual with celiac disease has a normal weight or is underweight.
**[[Obese]] and overweight individuals can have celiac disease.<ref name=pmid26892766>{{Cite journal  | last1 = Singh | first1 = I. | last2 = Agnihotri | first2 = A. | last3 = Sharma | first3 = A. | last4 = Verma | first4 = AK. | last5 = Das | first5 = P. | last6 = Thakur | first6 = B. | last7 = Sreenivas | first7 = V. | last8 = Gupta | first8 = SD. | last9 = Ahuja | first9 = V. | title = Patients with celiac disease may have normal weight or may even be overweight. | journal = Indian J Gastroenterol | volume =  | issue =  | pages =  | month = Feb | year = 2016 | doi = 10.1007/s12664-016-0620-9 | PMID = 26892766 }}</ref>


==Epidemiology==
===Epidemiology===
*Associated with:
Associated with:
**The skin condition ''[[dermatitis herpetiformis]]''.<ref>TN 2007 D22</ref>
*[[Dermatitis herpetiformis]] - skin condition.<ref name=Ref_TN2007_D22>{{Ref TN2007| D22}}</ref>
***Tx: dapsone.
**Tx: dapsone.
**IgA deficiency - 10-15X more common in celiac disease vs. healthy controls.<ref name=pmid12414763>{{Cite journal  | last1 = Kumar | first1 = V. | last2 = Jarzabek-Chorzelska | first2 = M. | last3 = Sulej | first3 = J. | last4 = Karnewska | first4 = K. | last5 = Farrell | first5 = T. | last6 = Jablonska | first6 = S. | title = Celiac disease and immunoglobulin a deficiency: how effective are the serological methods of diagnosis? | journal = Clin Diagn Lab Immunol | volume = 9 | issue = 6 | pages = 1295-300 | month = Nov | year = 2002 | doi =  | PMID = 12414763 }}</ref>
*IgA deficiency - 10-15X more common in celiac disease vs. healthy controls.<ref name=pmid12414763>{{Cite journal  | last1 = Kumar | first1 = V. | last2 = Jarzabek-Chorzelska | first2 = M. | last3 = Sulej | first3 = J. | last4 = Karnewska | first4 = K. | last5 = Farrell | first5 = T. | last6 = Jablonska | first6 = S. | title = Celiac disease and immunoglobulin a deficiency: how effective are the serological methods of diagnosis? | journal = Clin Diagn Lab Immunol | volume = 9 | issue = 6 | pages = 1295-300 | month = Nov | year = 2002 | doi =  | PMID = 12414763 }}</ref>
**Risk factor for ''gastrointestinal T cell lymphoma'' - known as: ''[[enteropathy-associated T cell lymphoma]]'' (EATL).
*Risk factor for ''gastrointestinal T cell lymphoma'' - known as: ''[[enteropathy-associated T cell lymphoma]]'' (EATL).
*[[IgA nephropathy]] is associated with an increased frequency of [[celiac disease]].<ref name=pmid19332868>{{Cite journal  | last1 = Smerud | first1 = HK. | last2 = Fellström | first2 = B. | last3 = Hällgren | first3 = R. | last4 = Osagie | first4 = S. | last5 = Venge | first5 = P. | last6 = Kristjánsson | first6 = G. | title = Gluten sensitivity in patients with IgA nephropathy. | journal = Nephrol Dial Transplant | volume = 24 | issue = 8 | pages = 2476-81 | month = Aug | year = 2009 | doi = 10.1093/ndt/gfp133 | PMID = 19332868 }}</ref>
*Esophageal [[squamous cell carcinoma]] - increased risk.<ref name=pmid11355914>{{Cite journal  | last1 = Messmann | first1 = H. | title = Squamous cell cancer of the oesophagus. | journal = Best Pract Res Clin Gastroenterol | volume = 15 | issue = 2 | pages = 249-65 | month = Apr | year = 2001 | doi = 10.1053/bega.2000.0172 | PMID = 11355914 }}</ref>
*Small bowel adenocarcinoma - increased risk.<ref name=pmid1060711>{{Cite journal  | last1 = West | first1 = RA. | last2 = McNeill | first2 = RW. | title = Maxillary alveolar hyperplasia, diagnosis and treatment planning. | journal = J Maxillofac Surg | volume = 3 | issue = 4 | pages = 239-50 | month = Dec | year = 1975 | doi =  | PMID = 1060711 }}</ref><ref name=pmid12940435>{{Cite journal  | last1 = Rampertab | first1 = SD. | last2 = Fleischauer | first2 = A. | last3 = Neugut | first3 = AI. | last4 = Green | first4 = PH. | title = Risk of duodenal adenoma in celiac disease. | journal = Scand J Gastroenterol | volume = 38 | issue = 8 | pages = 831-3 | month = Aug | year = 2003 | doi =  | PMID = 12940435 }}</ref>
*Thought to be related to the very rare [[collagenous sprue]] - controversial.<ref name=pmid21631278>{{Cite journal  | last1 = Zhao | first1 = X. | last2 = Johnson | first2 = RL. | title = Collagenous sprue: a rare, severe small-bowel malabsorptive disorder. | journal = Arch Pathol Lab Med | volume = 135 | issue = 6 | pages = 803-9 | month = Jun | year = 2011 | doi = 10.1043/2010-0028-RS.1 | PMID = 21631278 }}</ref><ref name=pmid23735026>{{Cite journal  | last1 = Busto-Bea | first1 = V. | last2 = Crespo-Pérez | first2 = L. | last3 = García-Miralles | first3 = N. | last4 = Ruiz-Del-Árbol-Olmos | first4 = L. | last5 = Cano-Ruiz | first5 = A. | title = Collagenous sprue: Don´t forget connective tissue in chronic diarrhea evaluation. | journal = Rev Esp Enferm Dig | volume = 105 | issue = 3 | pages = 171-174 | month = May | year = 2013 | doi =  | PMID = 23735026 }}</ref>
*[[Lymphocytic gastritis]] - seen in ~10% of individuals with celiac disease.<ref name=pmid9659261>{{Cite journal  | last1 = Feeley | first1 = KM. | last2 = Heneghan | first2 = MA. | last3 = Stevens | first3 = FM. | last4 = McCarthy | first4 = CF. | title = Lymphocytic gastritis and coeliac disease: evidence of a positive association. | journal = J Clin Pathol | volume = 51 | issue = 3 | pages = 207-10 | month = Mar | year = 1998 | doi =  | PMID = 9659261 }}</ref>


==Variants of celiac sprue==
==Variants of celiac sprue==
Line 18: Line 58:
#*NO villous arch. change.
#*NO villous arch. change.
#Refractory celiac sprue.
#Refractory celiac sprue.
#*Subclassified - see ''microscopic''.
#Collagenous sprue.
#Collagenous sprue.
#*Abundant mucosal collagen; see ''microscopic''.
#*Abundant mucosal collagen - see ''microscopic''.


==Clinical==
==Clinical==
Line 27: Line 68:


===Serologic testing===
===Serologic testing===
*Anti-transglutaminase antibody.
*Anti-tissue transglutaminase (TTG) antibody -- >10 U/mL considered positive.<ref>URL: [http://www.mayomedicallaboratories.com/test-catalog/Clinical+and+Interpretive/82587 http://www.mayomedicallaboratories.com/test-catalog/Clinical+and+Interpretive/82587]. Accessed on: 13 August 2012.</ref>
**Alternative test: anti-endomysial antibody.
**Alternative test: anti-endomysial antibody.
*IgA -- assoc. with celiac sprue.
*Anti-gliadin antibodies.<ref name=pmid19629760>{{Cite journal  | last1 = Matthias | first1 = T. | last2 = Pfeiffer | first2 = S. | last3 = Selmi | first3 = C. | last4 = Eric Gershwin | first4 = M. | title = Diagnostic challenges in celiac disease and the role of the tissue transglutaminase-neo-epitope. | journal = Clin Rev Allergy Immunol | volume = 38 | issue = 2-3 | pages = 298-301 | month = Apr | year = 2010 | doi = 10.1007/s12016-009-8160-z | PMID = 19629760 }}</ref>
*IgA deficiency - associated with celiac sprue.


==Microscopic==
==Microscopic==
Features:<ref name=Ref_PBoD843>{{Ref PBoD|843}}</ref>
Features:<ref name=Ref_PBoD843>{{Ref PBoD|843}}</ref>
*Intraepithelial lymphocytes - '''key feature'''.
*[[Intraepithelial lymphocytes]] (IELs) - '''key feature'''.
**Should be more pronounced at tips of villi.<ref name=pmid15280404>{{cite journal |author=Biagi F, Luinetti O, Campanella J, ''et al.'' |title=Intraepithelial lymphocytes in the villous tip: do they indicate potential coeliac disease? |journal=J. Clin. Pathol. |volume=57 |issue=8 |pages=835–9 |year=2004 |month=August |pmid=15280404 |pmc=1770380 |doi=10.1136/jcp.2003.013607 |url=}}</ref>
**Should be more pronounced at tips of villi.<ref name=pmid15280404>{{cite journal |author=Biagi F, Luinetti O, Campanella J, ''et al.'' |title=Intraepithelial lymphocytes in the villous tip: do they indicate potential coeliac disease? |journal=J. Clin. Pathol. |volume=57 |issue=8 |pages=835–9 |year=2004 |month=August |pmid=15280404 |pmc=1770380 |doi=10.1136/jcp.2003.013607 |url=}}</ref>
**Criteria for number varies:
*** > 40 IELs / 100 enterocytes (epithelial cells).<ref name=pmid10524652>{{cite journal |author=Oberhuber G, Granditsch G, Vogelsang H |title=The histopathology of coeliac disease: time for a standardized report scheme for pathologists |journal=Eur J Gastroenterol Hepatol |volume=11 |issue=10 |pages=1185–94 |year=1999 |month=October |pmid=10524652 |doi= |url=}}</ref>
*** > 25 IELs / 100 enterocytes (epithelial cells).<ref name=pmid17544877>{{cite journal |author=Corazza GR, Villanacci V, Zambelli C, ''et al.'' |title=Comparison of the interobserver reproducibility with different histologic criteria used in celiac disease |journal=Clin. Gastroenterol. Hepatol. |volume=5 |issue=7 |pages=838–43 |year=2007 |month=July |pmid=17544877 |doi=10.1016/j.cgh.2007.03.019 |url=}}</ref>
*Loss of villi - '''important feature'''.
*Loss of villi - '''important feature'''.
**Normal duodenal biopsy should have 3 good villi.
**Normal duodenal biopsy should have 3 good villi.
Line 41: Line 86:
*Mitosis increased (in the crypts).
*Mitosis increased (in the crypts).
*+/-Collagen band (pink material in mucosa) - "Collagenous sprue"; must encompass ~25% of mucosa.
*+/-Collagen band (pink material in mucosa) - "Collagenous sprue"; must encompass ~25% of mucosa.
Image:
*[http://commons.wikimedia.org/wiki/File:Coeliac_path.jpg Celiac sprue (WC)].


Notes:  
Notes:  
Line 50: Line 92:
*Flat lesions without IELs are unlikely to be celiac sprue.
*Flat lesions without IELs are unlikely to be celiac sprue.
*Mucosa erosions are rare in celiac sprue; should prompt consideration of an alternate diagnosis (infection, medications, Crohn's disease).
*Mucosa erosions are rare in celiac sprue; should prompt consideration of an alternate diagnosis (infection, medications, Crohn's disease).
*Biagi ''et al.''<ref name=pmid15280404>{{cite journal |author=Biagi F, Luinetti O, Campanella J, ''et al.'' |title=Intraepithelial lymphocytes in the villous tip: do they indicate potential coeliac disease? |journal=J. Clin. Pathol. |volume=57 |issue=8 |pages=835–9 |year=2004 |month=August |pmid=15280404 |pmc=1770380 |doi=10.1136/jcp.2003.013607 |url=}}</ref> count twenty cells in five (villi) tips.
===DDx===
*[[Giardiasis]].
**Have giardia organisms.
**Always consider ''Giardiasis'' and especially on exams.
*[[Crohn's disease]].
*[[Helicobacter gastritis]].<ref name=pmid16831028>{{Cite journal  | last1 = Brown | first1 = I. | last2 = Mino-Kenudson | first2 = M. | last3 = Deshpande | first3 = V. | last4 = Lauwers | first4 = GY. | title = Intraepithelial lymphocytosis in architecturally preserved proximal small intestinal mucosa: an increasing diagnostic problem with a wide differential diagnosis. | journal = Arch Pathol Lab Med | volume = 130 | issue = 7 | pages = 1020-5 | month = Jul | year = 2006 | doi = 10.1043/1543-2165(2006)130[1020:ILIAPP]2.0.CO;2 | PMID = 16831028 }}</ref>
*[[Cryptosporidiosis]].<ref name=pmid16831028/>
*[[Whipple's disease]] (very rare).
**Abundant macrophages should make one suspicious.
*[[Autoimmune enteropathy]] - pediatric population.
**Super duper rare.
*Drugs - e.g. olmesartan.<ref name=pmid24852741>{{Cite journal  | last1 = Fiorucci | first1 = G. | last2 = Puxeddu | first2 = E. | last3 = Colella | first3 = R. | last4 = Paolo Reboldi | first4 = G. | last5 = Villanacci | first5 = V. | last6 = Bassotti | first6 = G. | title = Severe spruelike enteropathy due to olmesartan. | journal = Rev Esp Enferm Dig | volume = 106 | issue = 2 | pages = 142-4 | month = Feb | year = 2014 | doi =  | PMID = 24852741 }}</ref>
*[[obesity|Morbid obesity]].<ref name=pmid17516730>{{Cite journal  | last1 = Harpaz | first1 = N. | last2 = Levi | first2 = GS. | last3 = Yurovitsky | first3 = A. | last4 = Kini | first4 = S. | title = Intraepithelial lymphocytosis in architecturally normal small intestinal mucosa: association with morbid obesity. | journal = Arch Pathol Lab Med | volume = 131 | issue = 3 | pages = 344; author reply 344 | month = Mar | year = 2007 | doi = 10.1043/1543-2165(2007)131[344b:IR]2.0.CO;2 | PMID = 17516730 }}</ref>
===Image===
<gallery>
Image: Celiac disease - low mag.jpg | CD - low mag. (WC/Nephron)
Image: Celiac disease - intermed mag.jpg | CD - intermed. mag. (WC/Nephron)
Image: Celiac disease - high mag.jpg | CD - high mag. (WC/Nephron)
Image: Celiac disease - very high mag.jpg | CD - very high mag. (WC/Nephron)
Image:Coeliac_path.jpg | Celiac sprue. (WC)
</gallery>
===Refractory sprue===
*Type I: CD3 ~= CD8.
*Type II: CD3 20% + less than CD8.


===Grading===
===Grading===
Most pathologists do not grade celiac sprue.
Many pathologists do not grade celiac sprue.


The most common system is the ''modified Marsh system'':<ref name=pmid12145668>{{cite journal |author=Wahab PJ, Meijer JW, Dumitra D, Goerres MS, Mulder CJ |title=Coeliac disease: more than villous atrophy |journal=Rom J Gastroenterol |volume=11 |issue=2 |pages=121–7 |year=2002 |month=June |pmid=12145668 |doi= |url=}}</ref><ref name=pmid20844959>{{cite journal |author=Ciaccio EJ, Bhagat G, Tennyson CA, Lewis SK, Hernandez L, Green PH |title=Quantitative Assessment of Endoscopic Images for Degree of Villous Atrophy in Celiac Disease |journal=Dig Dis Sci |volume= |issue= |pages= |year=2010 |month=September |pmid=20844959 |doi=10.1007/s10620-010-1371-6 |url=}}</ref>
====Marsh====
The ''Marsh system'', also ''Marsh-Oberhuber'':<ref name=pmid10524652>{{cite journal |author=Oberhuber G, Granditsch G, Vogelsang H |title=The histopathology of coeliac disease: time for a standardized report scheme for pathologists |journal=Eur J Gastroenterol Hepatol |volume=11 |issue=10 |pages=1185–94 |year=1999 |month=October |pmid=10524652 |doi= |url=}}</ref>
{| class="wikitable"
{| class="wikitable"
|
| Marsh score
| '''Marsh 1'''
| Descriptors
| '''Marsh 3A'''
| Villi
| '''Marsh 3C'''
| Intraepithelial <Br>lymphocytes (IELs)
| Crypts
|-
| Normal (Marsh 0)
| normal
| normal villi
| '''< 40 / 100 epithelial cells'''
| normal crypts
|-
| Marsh 1
| IELs increased
| normal villi
| '''> 40 / 100 epithelial cells'''
| '''normal crypts'''
|-
|-
| Marsh 2
| hypertrophic crypts, IELs
| '''normal villi'''
| > 40 / 100 epithelial cells
| '''hypertrophic crypts'''
|-
| Marsh 3a
| partial villous atrophy / blunted villi (mild)
| '''mild atrophy'''
| > 40 / 100 epithelial cells
| hypertrophic crypts
|-
| Marsh 3b
| moderate-to-marked villous atrophy /<br> blunted villi (moderate-to-marked)
| '''marked atrophy'''
| > 40 / 100 epithelial cells
| hypertrophic crypts
|-
| Marsh 3c
| total villous atrophy, flattened mucosa
| '''absent'''; flat as a pancake
| > 40 / 100 epithelial cells
| hypertrophic crypts
|}
====Simplified Marsh/Corazza====
A simplified Marsh system - based (only) on villous architecture:<ref name=pmid17544877>{{cite journal |author=Corazza GR, Villanacci V, Zambelli C, ''et al.'' |title=Comparison of the interobserver reproducibility with different histologic criteria used in celiac disease |journal=Clin. Gastroenterol. Hepatol. |volume=5 |issue=7 |pages=838–43 |year=2007 |month=July |pmid=17544877 |doi=10.1016/j.cgh.2007.03.019 |url=}}</ref>
{| class="wikitable"
| Grade
| Similar Marsh grade
| Descriptors
| Descriptors
| Well-formed villi
| Alternate descriptors
| Partial villous atrophy
|-
| Total villous atrophy
| A
| Marsh 1
| well-formed villi, IELs > 25/100 enterocytes
| normal villous architecture
|-
| B1
| Marsh 3a
| partial villous atrophy; villous-crypt ratio < 3:1
| blunted villi
|-
|-
| Alternate descriptors
| B2
| Normal villous arch.
| Marsh 3c
| Blunted villi
| total villous atrophy
| Flattened mucosa
| flattened mucosa
|}
|}


==DDx==
Notes:
*Giardiasis.
*Villous atrophy can be assessed endoscopically.<ref name=pmid20844959>{{cite journal |author=Ciaccio EJ, Bhagat G, Tennyson CA, Lewis SK, Hernandez L, Green PH |title=Quantitative Assessment of Endoscopic Images for Degree of Villous Atrophy in Celiac Disease |journal=Dig Dis Sci |volume= |issue= |pages= |year=2010 |month=September |pmid=20844959 |doi=10.1007/s10620-010-1371-6 |url=}}</ref>
**Have giarrdia organisms.
 
**Always consider ''Giardiasis'' and especially on exams.
==IHC==
*Whipple's disease (very rare).
*CD3 -- marks the IELs.<ref name=pmid15280404>{{cite journal |author=Biagi F, Luinetti O, Campanella J, ''et al.'' |title=Intraepithelial lymphocytes in the villous tip: do they indicate potential coeliac disease? |journal=J. Clin. Pathol. |volume=57 |issue=8 |pages=835–9 |year=2004 |month=August |pmid=15280404 |pmc=1770380 |doi=10.1136/jcp.2003.013607 |url=}}</ref>
**Abundant macrophages should make one suspicious.
 
==Sign out==
===TTG result not available===
<pre>
Duodenum, Biopsy:
- Small bowel mucosa with increased intraepithelial lymphocytes, villous
  architecture and crypt architecture within normal limits, see comment.
- Brunner's glands present.
- NEGATIVE for acute duodenitis.
- NEGATIVE for dysplasia.
 
Comment:
Focally, there are approximately 50 lymphocytes/100 enterocytes. Increased intraepithelial
lymphocytes is a nonspecific finding that may be seen in a number of conditions, including
infections (e.g. Helicobacter gastritis), obesity, and autoimmune disorders (e.g. Crohn's
disease).  It is seen in celiac disease; correlation with serology is suggested, if not done.
</pre>
 
====Block letters====
<pre>
SMALL BOWEL (DUODENUM), BIOPSY:
- SMALL BOWEL MUCOSA WITH INCREASED INTRAEPITHELIAL LYMPHOCYTES, VILLOUS
  ARCHITECTURE AND CRYPT ARCHITECTURE WITHIN NORMAL LIMITS, SEE COMMENT.
- NEGATIVE FOR ACUTE DUODENITIS.
- NEGATIVE FOR DYSPLASIA.
 
COMMENT:
There are approximately 45 lymphocytes/100 enterocytes. Increased intraepithelial
lymphocytes is a nonspecific finding. It is seen in celiac disease; correlation with TTG
serology is suggested, if not done.
</pre>
 
=====Alternate=====
<pre>
DUODENUM, BIOPSY:
- SMALL BOWEL MUCOSA WITH INCREASED INTRAEPITHELIAL LYMPHOCYTES, VILLOUS
  ARCHITECTURE AND CRYPT ARCHITECTURE WITHIN NORMAL LIMITS, SEE COMMENT.
- BRUNNER'S GLANDS PRESENT.
- NEGATIVE FOR ACUTE DUODENITIS.
- NEGATIVE FOR DYSPLASIA.
 
COMMENT:
Focally, there are approximately 50 lymphocytes/100 enterocytes. Increased intraepithelial
lymphocytes is a nonspecific finding that may be seen in a number of conditions, including
infections (e.g. Helicobacter gastritis), and autoimmune disorders (e.g. Crohn's disease). 
It is seen in celiac disease; correlation with TTG serology is suggested, if not done.
</pre>
 
===Positive TTG===
<pre>
DUODENUM, BIOPSY:
- SMALL BOWEL MUCOSA WITH BRUNNER'S GLANDS AND AN INCREASE OF INTRAEPITHELIAL LYMPHOCYTES
  (>50 LYMPHOCYTES/100 ENTEROCYTES), A PRESERVATION OF VILLOUS ARCHITECTURE AND CRYPTS
  WITHIN NORMAL LIMITS, SEE COMMENT.
 
COMMENT:
The findings are consistent with celiac disease, Marsh classification 1.
</pre>
 
<pre>
DUODENUM, BIOPSY:
- SMALL BOWEL MUCOSA WITH BRUNNER'S GLANDS, AN INCREASE OF INTRAEPITHELIAL LYMPHOCYTES
  (>60 LYMPHOCYTES/100 ENTEROCYTES), AND A BLUNTED VILLOUS ARCHITECTURE, SEE COMMENT.
 
COMMENT:
The findings are consistent with celiac disease, Marsh classification 3a.
</pre>
 
====Micro====
The sections show small bowel mucosa with Brunner's glands. Increased numbers of
intraepithelial lymphocytes are present ~ 50 lymphocytes/100 epithelial cells.
The villous architecture is within normal limits (no apparent villous blunting).
 
Neutrophils are present in the lamina propria; however, they are not found intraepithelial.
 
The epithelium matures normally to the surface (no dysplasia).


==See also==
==See also==
*[[Gastrointestinal pathology]].
*[[Gastrointestinal pathology]].
*[[Duodenum]].
*[[Duodenum]].
*[[Collagenous gastritis]].


==References==
==References==
Line 91: Line 289:


[[Category:Gastrointestinal pathology]]
[[Category:Gastrointestinal pathology]]
[[Category:Diagnosis]]
[[Category:Duodenum]]

Latest revision as of 12:50, 13 June 2019

Celiac sprue
Diagnosis in short

Celiac disease. H&E stain.
Synonyms celiac disease
LM Intraepithelial lymphocytes +/- villous blunting
LM DDx Giardiasis, Enteropathy-associated T-cell lymphoma (EATL), inflammatory bowel disease, MALT lymphoma, others
Site Duodenum
Associated Dx dermatitis herpetiformis, IgA deficiency, EATL, duodenal adenocarcinoma
Clinical history improves with gluten free diet
Signs diarrhea
Prevalence uncommon
Blood work TTG elevated (>10 U/mL)
Clin. DDx normal duodenum
Treatment gluten free diet

Celiac sprue, also celiac disease (abbreviated CD), is a common pathology that affects the duodenum.

It should not be confused with tropical sprue.

An introduction to gastrointestinal pathology is in the gastrointestinal pathology article. It covers basic gastrointestinal histology.

General

  • Considered an autoimmune disease.
  • The typical individual with celiac disease has a normal weight or is underweight.
    • Obese and overweight individuals can have celiac disease.[1]

Epidemiology

Associated with:

Variants of celiac sprue

  1. Latent celiac sprue.
    • ONLY intraepithelial lymphocytes.
    • NO villous arch. change.
  2. Refractory celiac sprue.
    • Subclassified - see microscopic.
  3. Collagenous sprue.
    • Abundant mucosal collagen - see microscopic.

Clinical

Treatment

  • Gluten free diet.
    • Mnemonic: BROW = barley, rye, oats, wheat.

Serologic testing

  • Anti-tissue transglutaminase (TTG) antibody -- >10 U/mL considered positive.[11]
    • Alternative test: anti-endomysial antibody.
  • Anti-gliadin antibodies.[12]
  • IgA deficiency - associated with celiac sprue.

Microscopic

Features:[13]

  • Intraepithelial lymphocytes (IELs) - key feature.
    • Should be more pronounced at tips of villi.[14]
    • Criteria for number varies:
      • > 40 IELs / 100 enterocytes (epithelial cells).[15]
      • > 25 IELs / 100 enterocytes (epithelial cells).[16]
  • Loss of villi - important feature.
    • Normal duodenal biopsy should have 3 good villi.
  • Plasma cells - abundant (weak feature).
  • Macrophages.
  • Mitosis increased (in the crypts).
  • +/-Collagen band (pink material in mucosa) - "Collagenous sprue"; must encompass ~25% of mucosa.

Notes:

  • If you see acute inflammatory cells, i.e. neutrophils, consider Giardiasis and other infectious etiologies.
  • Biopsy should consist of 2-3 sites. In children it is important to sample the duodenal cap, as it is the only affected site in ~10% of cases.
  • Flat lesions without IELs are unlikely to be celiac sprue.
  • Mucosa erosions are rare in celiac sprue; should prompt consideration of an alternate diagnosis (infection, medications, Crohn's disease).
  • Biagi et al.[14] count twenty cells in five (villi) tips.

DDx

Image

  • CD - low mag. (WC/Nephron)

  • CD - intermed. mag. (WC/Nephron)

  • CD - high mag. (WC/Nephron)

  • CD - very high mag. (WC/Nephron)

  • Celiac sprue. (WC)

Refractory sprue

  • Type I: CD3 ~= CD8.
  • Type II: CD3 20% + less than CD8.

Grading

Many pathologists do not grade celiac sprue.

Marsh

The Marsh system, also Marsh-Oberhuber:[15]

Marsh score Descriptors Villi Intraepithelial
lymphocytes (IELs) 		Crypts
Normal (Marsh 0) normal normal villi < 40 / 100 epithelial cells normal crypts
Marsh 1 IELs increased normal villi > 40 / 100 epithelial cells normal crypts
Marsh 2 hypertrophic crypts, IELs normal villi > 40 / 100 epithelial cells hypertrophic crypts
Marsh 3a partial villous atrophy / blunted villi (mild) mild atrophy > 40 / 100 epithelial cells hypertrophic crypts
Marsh 3b moderate-to-marked villous atrophy /
blunted villi (moderate-to-marked) 		marked atrophy > 40 / 100 epithelial cells hypertrophic crypts
Marsh 3c total villous atrophy, flattened mucosa absent; flat as a pancake > 40 / 100 epithelial cells hypertrophic crypts

Simplified Marsh/Corazza

A simplified Marsh system - based (only) on villous architecture:[16]

Grade Similar Marsh grade Descriptors Alternate descriptors
A Marsh 1 well-formed villi, IELs > 25/100 enterocytes normal villous architecture
B1 Marsh 3a partial villous atrophy; villous-crypt ratio < 3:1 blunted villi
B2 Marsh 3c total villous atrophy flattened mucosa

Notes:

  • Villous atrophy can be assessed endoscopically.[20]

IHC

  • CD3 -- marks the IELs.[14]

Sign out

TTG result not available

Duodenum, Biopsy:
- Small bowel mucosa with increased intraepithelial lymphocytes, villous
  architecture and crypt architecture within normal limits, see comment.
- Brunner's glands present.
- NEGATIVE for acute duodenitis.
- NEGATIVE for dysplasia.

Comment:
Focally, there are approximately 50 lymphocytes/100 enterocytes. Increased intraepithelial
lymphocytes is a nonspecific finding that may be seen in a number of conditions, including
infections (e.g. Helicobacter gastritis), obesity, and autoimmune disorders (e.g. Crohn's 
disease).  It is seen in celiac disease; correlation with serology is suggested, if not done.

Block letters

SMALL BOWEL (DUODENUM), BIOPSY:
- SMALL BOWEL MUCOSA WITH INCREASED INTRAEPITHELIAL LYMPHOCYTES, VILLOUS 
  ARCHITECTURE AND CRYPT ARCHITECTURE WITHIN NORMAL LIMITS, SEE COMMENT.
- NEGATIVE FOR ACUTE DUODENITIS.
- NEGATIVE FOR DYSPLASIA.

COMMENT:
There are approximately 45 lymphocytes/100 enterocytes. Increased intraepithelial
lymphocytes is a nonspecific finding. It is seen in celiac disease; correlation with TTG
serology is suggested, if not done.
Alternate
DUODENUM, BIOPSY:
- SMALL BOWEL MUCOSA WITH INCREASED INTRAEPITHELIAL LYMPHOCYTES, VILLOUS
  ARCHITECTURE AND CRYPT ARCHITECTURE WITHIN NORMAL LIMITS, SEE COMMENT.
- BRUNNER'S GLANDS PRESENT.
- NEGATIVE FOR ACUTE DUODENITIS.
- NEGATIVE FOR DYSPLASIA.

COMMENT:
Focally, there are approximately 50 lymphocytes/100 enterocytes. Increased intraepithelial
lymphocytes is a nonspecific finding that may be seen in a number of conditions, including
infections (e.g. Helicobacter gastritis), and autoimmune disorders (e.g. Crohn's disease).  
It is seen in celiac disease; correlation with TTG serology is suggested, if not done.

Positive TTG

DUODENUM, BIOPSY: 
- SMALL BOWEL MUCOSA WITH BRUNNER'S GLANDS AND AN INCREASE OF INTRAEPITHELIAL LYMPHOCYTES 
  (>50 LYMPHOCYTES/100 ENTEROCYTES), A PRESERVATION OF VILLOUS ARCHITECTURE AND CRYPTS 
  WITHIN NORMAL LIMITS, SEE COMMENT. 

COMMENT: 
The findings are consistent with celiac disease, Marsh classification 1.

DUODENUM, BIOPSY: 
- SMALL BOWEL MUCOSA WITH BRUNNER'S GLANDS, AN INCREASE OF INTRAEPITHELIAL LYMPHOCYTES 
  (>60 LYMPHOCYTES/100 ENTEROCYTES), AND A BLUNTED VILLOUS ARCHITECTURE, SEE COMMENT. 

COMMENT: 
The findings are consistent with celiac disease, Marsh classification 3a.

Micro

The sections show small bowel mucosa with Brunner's glands. Increased numbers of intraepithelial lymphocytes are present ~ 50 lymphocytes/100 epithelial cells. The villous architecture is within normal limits (no apparent villous blunting).

Neutrophils are present in the lamina propria; however, they are not found intraepithelial.

The epithelium matures normally to the surface (no dysplasia).

See also

References

  1. ↑ Singh, I.; Agnihotri, A.; Sharma, A.; Verma, AK.; Das, P.; Thakur, B.; Sreenivas, V.; Gupta, SD. et al. (Feb 2016). "Patients with celiac disease may have normal weight or may even be overweight.". Indian J Gastroenterol. doi:10.1007/s12664-016-0620-9. PMID 26892766.
  2. ↑ Greenwald, J.; Heng, M. (2007). Toronto Notes for Medical Students 2007 (2007 ed.). The Toronto Notes Inc. for Medical Students Inc.. pp. D22. ISBN 978-0968592878.
  3. ↑ Kumar, V.; Jarzabek-Chorzelska, M.; Sulej, J.; Karnewska, K.; Farrell, T.; Jablonska, S. (Nov 2002). "Celiac disease and immunoglobulin a deficiency: how effective are the serological methods of diagnosis?". Clin Diagn Lab Immunol 9 (6): 1295-300. PMID 12414763.
  4. ↑ Smerud, HK.; Fellström, B.; Hällgren, R.; Osagie, S.; Venge, P.; Kristjánsson, G. (Aug 2009). "Gluten sensitivity in patients with IgA nephropathy.". Nephrol Dial Transplant 24 (8): 2476-81. doi:10.1093/ndt/gfp133. PMID 19332868.
  5. ↑ Messmann, H. (Apr 2001). "Squamous cell cancer of the oesophagus.". Best Pract Res Clin Gastroenterol 15 (2): 249-65. doi:10.1053/bega.2000.0172. PMID 11355914.
  6. ↑ West, RA.; McNeill, RW. (Dec 1975). "Maxillary alveolar hyperplasia, diagnosis and treatment planning.". J Maxillofac Surg 3 (4): 239-50. PMID 1060711.
  7. ↑ Rampertab, SD.; Fleischauer, A.; Neugut, AI.; Green, PH. (Aug 2003). "Risk of duodenal adenoma in celiac disease.". Scand J Gastroenterol 38 (8): 831-3. PMID 12940435.
  8. ↑ Zhao, X.; Johnson, RL. (Jun 2011). "Collagenous sprue: a rare, severe small-bowel malabsorptive disorder.". Arch Pathol Lab Med 135 (6): 803-9. doi:10.1043/2010-0028-RS.1. PMID 21631278.
  9. ↑ Busto-Bea, V.; Crespo-Pérez, L.; García-Miralles, N.; Ruiz-Del-Árbol-Olmos, L.; Cano-Ruiz, A. (May 2013). "Collagenous sprue: Don´t forget connective tissue in chronic diarrhea evaluation.". Rev Esp Enferm Dig 105 (3): 171-174. PMID 23735026.
  10. ↑ Feeley, KM.; Heneghan, MA.; Stevens, FM.; McCarthy, CF. (Mar 1998). "Lymphocytic gastritis and coeliac disease: evidence of a positive association.". J Clin Pathol 51 (3): 207-10. PMID 9659261.
  11. ↑ URL: http://www.mayomedicallaboratories.com/test-catalog/Clinical+and+Interpretive/82587. Accessed on: 13 August 2012.
  12. ↑ Matthias, T.; Pfeiffer, S.; Selmi, C.; Eric Gershwin, M. (Apr 2010). "Diagnostic challenges in celiac disease and the role of the tissue transglutaminase-neo-epitope.". Clin Rev Allergy Immunol 38 (2-3): 298-301. doi:10.1007/s12016-009-8160-z. PMID 19629760.
  13. ↑ Cotran, Ramzi S.; Kumar, Vinay; Fausto, Nelson; Nelso Fausto; Robbins, Stanley L.; Abbas, Abul K. (2005). Robbins and Cotran pathologic basis of disease (7th ed.). St. Louis, Mo: Elsevier Saunders. pp. 843. ISBN 0-7216-0187-1.
  14. ↑ 14.0 14.1 14.2 Biagi F, Luinetti O, Campanella J, et al. (August 2004). "Intraepithelial lymphocytes in the villous tip: do they indicate potential coeliac disease?". J. Clin. Pathol. 57 (8): 835–9. doi:10.1136/jcp.2003.013607. PMC 1770380. PMID 15280404. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1770380/.
  15. ↑ 15.0 15.1 Oberhuber G, Granditsch G, Vogelsang H (October 1999). "The histopathology of coeliac disease: time for a standardized report scheme for pathologists". Eur J Gastroenterol Hepatol 11 (10): 1185–94. PMID 10524652.
  16. ↑ 16.0 16.1 Corazza GR, Villanacci V, Zambelli C, et al. (July 2007). "Comparison of the interobserver reproducibility with different histologic criteria used in celiac disease". Clin. Gastroenterol. Hepatol. 5 (7): 838–43. doi:10.1016/j.cgh.2007.03.019. PMID 17544877.
  17. ↑ 17.0 17.1 Brown, I.; Mino-Kenudson, M.; Deshpande, V.; Lauwers, GY. (Jul 2006). "Intraepithelial lymphocytosis in architecturally preserved proximal small intestinal mucosa: an increasing diagnostic problem with a wide differential diagnosis.". Arch Pathol Lab Med 130 (7): 1020-5. doi:10.1043/1543-2165(2006)130[1020:ILIAPP]2.0.CO;2. PMID 16831028.
  18. ↑ Fiorucci, G.; Puxeddu, E.; Colella, R.; Paolo Reboldi, G.; Villanacci, V.; Bassotti, G. (Feb 2014). "Severe spruelike enteropathy due to olmesartan.". Rev Esp Enferm Dig 106 (2): 142-4. PMID 24852741.
  19. ↑ Harpaz, N.; Levi, GS.; Yurovitsky, A.; Kini, S. (Mar 2007). "Intraepithelial lymphocytosis in architecturally normal small intestinal mucosa: association with morbid obesity.". Arch Pathol Lab Med 131 (3): 344; author reply 344. doi:10.1043/1543-2165(2007)131[344b:IR]2.0.CO;2. PMID 17516730.
  20. ↑ Ciaccio EJ, Bhagat G, Tennyson CA, Lewis SK, Hernandez L, Green PH (September 2010). "Quantitative Assessment of Endoscopic Images for Degree of Villous Atrophy in Celiac Disease". Dig Dis Sci. doi:10.1007/s10620-010-1371-6. PMID 20844959.

External links

Review article(s)

Retrieved from "https://librepathology.org/w/index.php?title=Celiac_sprue&oldid=50249"