Difference between revisions of "Trophoblast"
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**Clear cytoplasm. | **Clear cytoplasm. | ||
**Single uniform nucleus. | **Single uniform nucleus. | ||
===Implantation site=== | |||
*Intermediate trophoblast. | |||
====IHC==== | |||
Features:<ref name=pmid1694585>{{cite journal |author=Yeh IT, O'Connor DM, Kurman RJ |title=Intermediate trophoblast: further immunocytochemical characterization |journal=Mod. Pathol. |volume=3 |issue=3 |pages=282–7 |year=1990 |month=May |pmid=1694585 |doi= |url=}}</ref> | |||
*hPL (human placental lactogen) +ve. | |||
*beta-hCG -ve. | |||
*Keratin +ve. | |||
*EMA +ve in 2nd & 3rd trimester. | |||
Notes: | |||
*Cytotrophoblast and syncytiotrophoblast - keratin +ve.<ref name=pmid1694585/> | |||
==Age of villi== | ==Age of villi== | ||
Revision as of 12:56, 22 July 2010
Chorionic villi are part of a normal pregnancy. They are derived from the conceptus, i.e. NOT maternal.
Tumours arising from the trophoblast are dealt with in gestational trophoblastic disease.
Chorionic villi 101
- Maternal blood is around villi.
- Fetal blood (nucleated (fetal) RBCs) in the villi.
Basic histology
- Outer layer of villus: syncytiotrophoblasts.
- Beneath syncytiotrophoblast (and closer to fetus) cytotrophoblasts.
Chorionic villi 102
- Syncytiotrophoblasts (eosinophilic cytoplasm with vacuoles (contain hCG), multiple hyperchromatic nuclei).
- Large + many irreg. or lobular hyperchromatic nuclei.
- Eosinophilic vacuolated cytoplasm (contains hCG).
- Closest to mom - covers cytotrophoblast.[1]
- Cytotrophoblasts (polygonal shape, distinct borders, clear cytoplasm, in cords, single nucleus).
- Polygonal shaped cells in cords/masses.
- Distinct cell borders.
- Covered by syncytiobrophoblast[2] - closer to fetus than syncytiotrophoblasts.
- Clear cytoplasm.
- Single uniform nucleus.
Implantation site
- Intermediate trophoblast.
IHC
Features:[3]
- hPL (human placental lactogen) +ve.
- beta-hCG -ve.
- Keratin +ve.
- EMA +ve in 2nd & 3rd trimester.
Notes:
- Cytotrophoblast and syncytiotrophoblast - keratin +ve.[3]
Age of villi
Young villi
- Do not have blood vessels.
- No syncytial knots.
Old villi
- Blood vessels present and at the periphery of villus - contain nucleated (fetal) RBCs.
- Syncytial knots.
See also
References
- ↑ http://upload.wikimedia.org/wikipedia/commons/4/45/Gray37.png
- ↑ http://upload.wikimedia.org/wikipedia/commons/4/45/Gray37.png
- ↑ 3.0 3.1 Yeh IT, O'Connor DM, Kurman RJ (May 1990). "Intermediate trophoblast: further immunocytochemical characterization". Mod. Pathol. 3 (3): 282–7. PMID 1694585.
