Difference between revisions of "Tissue processing"
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==See also== | ==See also== | ||
*[[Tissue fixation]]. | *[[Tissue fixation]]. | ||
*[[Molecular pathology]]. | |||
==References== | ==References== | ||
Revision as of 13:28, 4 May 2011
Tissue processing is a key component of the histologic examination. This article covers it superficially. It is something a pathologist should have a rudimentary understanding of.
What happens to the tissue
In an ideal world the pathologist gets the tissue fresh and divides it up in the following way:[1]
- Formulin.
- Electron microscopy.
- Molecular genetics.
- Cytogenetics.
- Flow cytometery.
- Tissue banks - institutional, regional, national, international.
- Tissue culture.
Tissue processing
Basic process:
- Fixation -- formulin
- Embedding -- replace water (in the tissue) with (paraffin) wax - using a series of alcohols.
- Sectioning -- cut with microtome ~ 3-10 micrometers thick, routinue is usu. 6-8 micrometers.[2]
- Staining.
See also
References
- ↑ PST. 14 February 2011.
- ↑ URL: http://library.med.utah.edu/WebPath/HISTHTML/HISTOTCH/HISTOTCH.html. Accessed on: 7 March 2011.